Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10841] to COUP TF1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free
    See all COUP TF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10841] to COUP TF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, Human fetal kidney and 293 cell lysates; Human glioblastoma tissue, Human transitional cell carcinoma tissue, and 293 cells.

  • General notes

    Ab224272 is the carrier-free version of ab181137. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab224272 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling COUP TF1 with Purified ab181137 at 1:100 dilution. Heat mediated antigen retrieval was performed using citrate buffer, pH6.0 . Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).

  • Flow Cytometry - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Flow Cytometry - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Flow Cytometry analysis of MCF7 (Human cervix adenocarcinoma epithelial cell) cells labeling COUP TF1 with purified ab181137 at 1:500 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).

  • Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Coup TF2 with Purified ab181137 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).

  • Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7cells labelling COUP TF1 with unpurified ab181137 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Immunohistochemical analysis of paraffin-embedded Human glioblastoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).

  • Flow Cytometry - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Flow Cytometry - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Flow cytometric analysis of COUP TF1 labeling 2% paraformaldehyde fixed 293 cells using unpurified ab181137 at 1/70 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    This IHC data was generated using the same anti-COUP TF1 antibody clone [EPR10841] in a different buffer formulation (cat# ab181137).

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma staining COUP TF1 using ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.

  • Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
    Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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