Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)
![Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)](https://www.abcam.com/ps/products/210/ab210038/Images/ab210038-257297-ICCap00259991.jpg "Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR10841] to COUP TF1
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841]
See all COUP TF1 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR10841] to COUP TF1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10841 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)
ab210038 staining COUP TF1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210038 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
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![Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)](https://www.abcam.com/ps/products/210/ab210038/Images/ab210038-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)
