Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)
![Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)](https://www.abcam.com/ps/products/195/ab195467/Images/ab195467-249938-anti-histone-h2a-crotonyl-k119-antibody-epr18769-dot-blot.jpg "Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18769] to Histone H2A (crotonyl K119)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Histone H2A (crotonyl K119) antibody [EPR18769]
See all Histone H2A primary antibodies -
Description
Rabbit monoclonal [EPR18769] to Histone H2A (crotonyl K119) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18769 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)
Dot blot analysis of H2A K119 crotonyl peptide (Lane 1), H2A K119 ubiquitinate peptide (Lane 2), H2A.X K119 ubiquitinate peptide (Lane 3), H2A K118 formyl peptide (Lane 4), H2A.X unmodified peptide (Lane 5) and H2A unmodified peptide (Lane 6), labeled using ab195467 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H+L (HRP) (ab97051) secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% BSA/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)](https://www.abcam.com/ps/products/195/ab195467/Images/ab195467-249936-anti-histone-h2a-crotonyl-k119-antibody-epr18769-western-blot.jpg)
Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% BSA/TBST.
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![Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)](https://www.abcam.com/ps/products/195/ab195467/Images/ab195467-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)
