Anti-Histone H2A (crotonyl K118) antibody [EP18768] (ab195466)
Key features and details
- Rabbit monoclonal [EP18768] to Histone H2A (crotonyl K118)
- Suitable for: WB, PepArr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2A (crotonyl K118) antibody [EP18768]
See all Histone H2A primary antibodies -
Description
Rabbit monoclonal [EP18768] to Histone H2A (crotonyl K118) -
Host species
Rabbit -
Tested applications
Suitable for: WB, PepArrmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and NIH/3T3 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP18768 -
Isotype
IgG -
Research areas
Images
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Anti-Histone H2A (crotonyl K118) antibody [EP18768] (ab195466) at 1/500 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% BSA/TBST.
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Anti-Histone H2A (crotonyl K118) antibody [EP18768] (ab195466) at 1/500 dilution + NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 2 secondsBlocking/Dilution buffer: 5% BSA/TBST.
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ab195466 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.