Antibodies, Proteins, Kits and Reagents for Life Science

Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17353] to p53 (phospho S37)
Suitable for: Dot blot, WB
Reacts with: Human

Product name

Anti-p53 (phospho S37) antibody [EPR17353]
See all p53 primary antibodies
Description

Rabbit monoclonal [EPR17353] to p53 (phospho S37)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- p53 transfected HEK-293 whole cell lysate treated with UV (60 J/M2) for 2 hours; HEK-293 and A431 whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17353
Isotype

IgG
Research areas

Western blot - Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/1000 dilution

Lane 1 : A431 whole cell lysate
Lane 2 : A431 treated with 1µg/ml doxorubicin for 24 hours whole cell lysate
Lane 3 : A431 treated with 1µg/ml doxorubicin for 24 hours whole cell lysate, the membrane was incubated with alkaline phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 53 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Dilution and blocking buffer: 5% NFDM/TBST.
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/50000 dilution

Lane 1 : p53 transfected HEK-293 whole cell lysate (Human epithelial cells from embryonic kidney)
Lane 2 : p53 transfected HEK-293 whole cell lysate treated with UV (60 J/M2) for 2 hours

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/5000 dilution

Lane 1 : A431 whole cell lysate (Human epidermoid carcinoma)
Lane 2 : A431 whole cell lysate treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

Dot blot analysis of p53 (phospho S37) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab182164 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/5000 dilution

Lane 1 : HEK-293 whole cell lysate (Human epithelial cells from embryonic kidney)
Lane 2 : HEK-293 whole cell lysate treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/1000 dilution + Saos-2 whole cell lysate (Human osteosarcoma cell line) at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 43 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Saos-2 doesn't express p53.
Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)

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