Anti-p53 (phospho S37) antibody [EPR17353] (ab182164)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17353] to p53 (phospho S37)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
-
Product name
Anti-p53 (phospho S37) antibody [EPR17353]
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR17353] to p53 (phospho S37) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- p53 transfected HEK-293 whole cell lysate treated with UV (60 J/M2) for 2 hours; HEK-293 and A431 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17353 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : A431 treated with 1µg/ml doxorubicin for 24 hours whole cell lysate
Lane 3 : A431 treated with 1µg/ml doxorubicin for 24 hours whole cell lysate, the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsDilution and blocking buffer: 5% NFDM/TBST.
-
All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/50000 dilution
Lane 1 : p53 transfected HEK-293 whole cell lysate (Human epithelial cells from embryonic kidney)
Lane 2 : p53 transfected HEK-293 whole cell lysate treated with UV (60 J/M2) for 2 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/5000 dilution
Lane 1 : A431 whole cell lysate (Human epidermoid carcinoma)
Lane 2 : A431 whole cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
Dot blot analysis of p53 (phospho S37) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab182164 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
-
All lanes : Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/5000 dilution
Lane 1 : HEK-293 whole cell lysate (Human epithelial cells from embryonic kidney)
Lane 2 : HEK-293 whole cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
Anti-p53 (phospho S37) antibody [EPR17353] (ab182164) at 1/1000 dilution + Saos-2 whole cell lysate (Human osteosarcoma cell line) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Saos-2 doesn't express p53.
-