Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase
Suitable for: WB, Flow Cyt, ICC, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Tyrosine Hydroxylase antibody [EP1533Y]
See all Tyrosine Hydroxylase primary antibodies
Description

Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Mouse
IHC-P	Mouse
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab183303)
Positive control
- WB: Human adrenal gland lysate. SH-SY5Y whole cell lysate. Mouse and rat brain lysate. PC-12 whole cell lysate. IHC-P: Human and mouse cerebral cortex tissue, Rat and mouse brain tissue. ICC: Neuro-2a and mouse midbrain neuron cells. Flow Cyt: SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1533Y
Isotype

IgG
Research areas

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

All lanes : Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) at 1/1000 dilution (Purified)

Lane 1 : Human adrenal gland lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) This image is courtesy of an Abreview submitted by Carl Hobbs

Immunohistochemical analysis of rat brain tissue section labeling Tyrosine Hydroxylase with ab75875 at 1/1000 dilution. The tissue was fixed with Formaldehyde. Antigen retrieval was heat mediated using citric acid. A biotin conjugated goat anti-rabbit polyclonal IgG at 1/250 was used as the secondary antibody.
See Abreview
Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse midbrain neuron cells labelling Tyrosine Hydroxylase with ab75875 at 1/10 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary midbrain neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).
Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/50 dilution (2.18 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue sections labeling Tyrosine Hydroxylase with purified ab75875 at 1/1500 dilution (0.07 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) This image is courtesy of an Abreview submitted by Carl Hobbs.

Immunohistochemical analysis of mouse brain tissue section labeling Tyrosine Hydroxylase with ab75875 at 1/800 dilution. The tissue was fixed with Formaldehyde. Antigen retrieval was heat mediated using citric acid. A biotin conjugated goat anti-rabbit polyclonal IgG at 1/250 was used as the secondary antibody.
See Abreview
Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

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