Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)
![Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)](https://www.abcam.com/ps/products/200/ab200547/Images/ab200547-456602-anti-cytokeratin-8-immunohistochemistry-lung-carcinoma-human.jpg "Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)")
Key features and details
- Mouse monoclonal [H1-TS1] to Cytokeratin 8
- Suitable for: Flow Cyt, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 8 antibody [H1-TS1]
See all Cytokeratin 8 primary antibodies -
Description
Mouse monoclonal [H1-TS1] to Cytokeratin 8 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanIHC-P Human
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Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 8. Cytoskeleton preparation containing cytokeratin 8 (H1); Keratin preparation from a human carcinoma (TS1)
Database link: P05787 -
Positive control
- IHC: Human lung carcinoma and colon carcinoma tissues Flow Cyt: Human HeLa cells ICC: Human HeLa and HCT116 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
H1-TS1 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Cytokeratin 8 with ab200547 at 2µg/ml dilution. Heat mediated antigen retrieval performed with tris/EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)](https://www.abcam.com/ps/products/200/ab200547/Images/ab200547-456601-anti-cytokeratin-8-immunocytochemistry-hela-cells-human.jpg)
Immunocytochemistry - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)Immunocytochemistry immunofluorescence analysis of HeLa cells (human epithelial cell line from cervix adenocarcinoma) stained with ab200547 at 2µg/ml (green) and nuclear counterstaining with RedDot (red)
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![Immunocytochemistry - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)](https://www.abcam.com/ps/products/200/ab200547/Images/ab200547-456600-anti-cytokeratin-8-immunocytochemistry-hct116-cells-human.jpg)
Immunocytochemistry - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)Immunocytochemistry immunofluorescence analysis of HCT116 cells (human colorectal carcinoma cell line) stained with ab200547 at 2µg/ml (green) and nuclear counterstaining with RedDot (red)
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![Flow Cytometry - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)](https://www.abcam.com/ps/products/200/ab200547/Images/ab200547-456599-anti-cytokeratin-8-flow-cytometry-hela-cells-human.jpg)
Flow Cytometry - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)Flow cytometry analysis of Hela cells (human epithelial cell line from cervix adenocarcinoma) stained with ab200547 (blue) and an isotype control (red)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)](https://www.abcam.com/ps/products/200/ab200547/Images/ab200547-243018-anti-cytokeratin-8-antibody-h1-ts1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [H1-TS1] (ab200547)Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma tissue labeling Cytokeratin 8 with ab200547 at 1 μg/mL.
