Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP1628Y] to Cytokeratin 8
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y]
    See all Cytokeratin 8 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP1628Y] to Cytokeratin 8

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
    Flow Cytometry - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468) Shi Q et al tem Cell Res Ther. 2017 Nov 2;8(1):246. doi: 10.1186/s13287-017-0700-5. Fig 5a. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    H89 blocks the 8-Br-cAMP-induced differentiation of WJ-MSCs into ESC-like cells.

    (Panel A) Flow cytometric analysis of cells in the five groups using antibodies against vimentin and CD13.

    (a) no inhibition group; (b) p38 MAPK-block group; (c) ERK1/2-block group; (d) PKA-block group; (e) no differentiation group. Cells in Groups a–d treated with 8-Br-cAMP for 21 days.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)

    ab192468 staining Cytokeratin 8 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192468 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor® 488 Goat anti-mouse IgG (H&L - preadsorbed) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells tested under the same conditions.

    Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8).

  • Flow Cytometry - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
    Flow Cytometry - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)

    Overlay histogram showing HeLa cells stained with ab192468 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192468, 2μl/1x106) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Alexa Fluor® 647) (2μl/1x106) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 25mW red solid state diode laser (635nm) and 675/30 bandpass filter.

    This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

  • Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
    Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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