Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸ 1 ml 465 705 ₸

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Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP102] to Cytokeratin 8 - C-terminal
Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytokeratin 8 antibody [SP102] - C-terminal
See all Cytokeratin 8 primary antibodies
Description

Rabbit monoclonal [SP102] to Cytokeratin 8 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-Fr	Rat
IHC-P	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast ductal carcinoma, breast, lung squamous cell carcinoma, placenta, thyroid, bladder, bladder transitional cell carcinoma, stomach, stomach adenocarcinoma, prostate, prostate adenocarcinoma and lung tissues. Flow Cyt: HaCaT cells. IHC-Fr: Rat and mouse small intestine Tissue ICC/IF: HaCaT cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP102
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat small intestine tissue sections labeling Cytokeratin 8 with Purified ab227644 at 1:20 (6.85 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1:25 (5.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse small intestine tissue sections labeling Cytokeratin 8 with Purified ab227644 at 1:20 (6.85 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
Flow Cytometry - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Flow Cytometry analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1:200 dilution (0.685 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.
Anti-Cytokeratin 8 antibody [SP102] - C-terminal (ab227644)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com