Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free (ab271843)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1374Y] to Apolipoprotein E - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB, IHC-P, ICC
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free
    See all Apolipoprotein E primary antibodies

  • Description

    Rabbit monoclonal [EP1374Y] to Apolipoprotein E - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • human normal liver tissue; HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab271843 is the carrier-free version of ab52607. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Immunocytochemistry - Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free (ab271843)
    Immunocytochemistry - Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free (ab271843)

    This data was developed using ab52607, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein E with Purified ab52607 at 1:50 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free (ab271843)
    Flow Cytometry - Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free (ab271843)

    Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein E (red) with purified ab52607 at a 1/250 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52607).
  • Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free (ab271843)
    Anti-Apolipoprotein E antibody [EP1374Y] - BSA and Azide free (ab271843)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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