Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19392] to Apolipoprotein E - Low endotoxin, Azide free
  • Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free
    See all Apolipoprotein E primary antibodies

  • Description

    Rabbit monoclonal [EPR19392] to Apolipoprotein E - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver and fetal kidney lysates; Rat and mouse liver lysates; HepG2 whole cell lysate; Human, mouse and rat plasma; Mouse brain and heart lysates; Rat brain and kidney lysates. IHC-P: Mouse liver and thalamus tissues; Rat liver and cerebral cortex tissues; Human liver and tonsil tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: Mouse plasma.

  • General notes

    ab227993 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Immunohistochemical analysis of paraffin-embedded Mouse thalamus tissue labeling Apolipoprotein E with ab183597 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on astrocytes of mouse thalamus is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Apolipoprotein E with ab183597 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatocytes of rat liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Apolipoprotein E with ab183597 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on astrocytes of rat cerebral cortex is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Apolipoprotein E with ab183597 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatocytes of Human liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Apolipoprotein E with ab183597 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophages of Human tonsil is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Immunofluorescent analysis of 100% methanol-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Apolipoprotein E with ab183597 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab183597 at 1/500 dilution followed by ab150120  at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

  • Flow Cytometry - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Flow Cytometry - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Apolipoprotein E with ab183597 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

  • Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    Apolipoprotein E was immunoprecipitated from 1mg of Mouse plasma with ab183597 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183597 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse plasma, 10µg (Input).

    Lane 2: ab183597 IP in Mouse plasma.

    Lane 3: Rabbit IgG,monoclonal[EPR25A] - Isotype Control (ab172730) instead of ab183597 in Mouse plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183597).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

    This IHC data was generated using the same anti-Apolipoprotein E antibody clone, EPR19392, in a different buffer formulation (cat# ab183597).

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Apolipoprotein E with ab183597 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatocytes of mouse liver, and plasma was also stained. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)
    Anti-Apolipoprotein E antibody [EPR19392] - Low endotoxin, Azide free (ab227993)

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