Anti-hnRNP K antibody [3C2] (ab39975)
![Anti-hnRNP K antibody [3C2] (ab39975)](https://www.abcam.com/ps/products/39/ab39975/Images/ab39975-53334-anti-hnrnp-k-antibody-3c2-chip-grade-western-blot.jpg "Anti-hnRNP K antibody [3C2] (ab39975)")
Key features and details
- Mouse monoclonal [3C2] to hnRNP K
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-hnRNP K antibody [3C2]
See all hnRNP K primary antibodies -
Description
Mouse monoclonal [3C2] to hnRNP K -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Full length native protein (purified) (Human)
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Positive control
- HeLa extracts. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3C2 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-hnRNP K antibody [3C2] (ab39975)All lanes : Anti-hnRNP K antibody [3C2] (ab39975) at 1/1000 dilution
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible post-translational modification)
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![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [3C2] (ab39975)](https://www.abcam.com/ps/products/39/ab39975/Images/ab39975-172576-anti-hnrnp-k-antibody-3c2-chip-grade-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [3C2] (ab39975)ICC/IF image of ab39975 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39975 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-hnRNP K antibody [3C2] (ab39975)](https://www.abcam.com/ps/products/39/ab39975/Images/ab39975-53333-anti-hnrnp-k-antibody-3c2-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-hnRNP K antibody [3C2] (ab39975)Overlay histogram showing HeLa cells stained with ab39975 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab39975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
