ALT Assay Kit (384 well, Colorimetric/Fluorometric) (ab241035)
Key features and details
- Detection method: Colorimetric/Fluorometric
Overview
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Product name
ALT Assay Kit (384 well, Colorimetric/Fluorometric)
See all Alanine Transaminase kits -
Detection method
Colorimetric/Fluorometric -
Product overview
The ALT Assay Kit (384 well, Colorimetric/Fluorometric) (ab241035) provides a rapid, sensitive and reliable test, suitable for high throughput screening of ALT activity in various biological samples.
The 384-well format allows for the screening of a large number of samples on a single high-density microplate. The kit can be run in either colorimetric or fluorometric detection mode and can detect a minimum of 0.25 to 1.25 mU of ALT activity per well, using a minimal sample volume as low as 0.5 μL.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 400 tests ALT Assay Buffer 1 x 25ml ALT Enzyme Mix 1 vial ALT Positive Control 1 vial ALT Substrate 1 vial Probe 1 x 0.2ml Pyruvate Standard 1 x 0.1ml -
Research areas
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Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. -
Tissue specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. -
Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AAT1
- Alanine aminotransferase
- Alanine aminotransferase 1
see all
Images
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Pyruvate Standard Curve (colorimetric).Pyruvate Standard Curve (colorimetric).
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Pyruvate Standard Curve (fluorometric).Pyruvate Standard Curve (fluorometric). -
ALT Activity (mU/ml) in 3T3 Cell Lysate.ALT Activity (mU/mL) in 3T3 Cell Lysate (2.5 µL, 12 mg/mL protein). Pooled Human Serum (2.5 µL), and Jurkat Cell Lysate (1.25 µL, 4 mg/ml protein). Assays were performed according to the kit protocol.
