Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

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Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1373Y] to Apolipoprotein E
Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Apolipoprotein E antibody [EP1373Y]
See all Apolipoprotein E primary antibodies
Description

Rabbit monoclonal [EP1373Y] to Apolipoprotein E
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

within Human Apolipoprotein E aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: HeLa cell lysate; Human serum, fetal liver and liver cancer tissue lysate. IHC-P: Human liver and fetal liver tissues. ICC/IF: HepG2 cells. Flow Cyt: 293T cells. IP: Human fetal liver tissue lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1373Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling Apolipoprotein with unpurified ab51015 at 1/100.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Apolipoprotein E with purified ab51015 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Flow Cytometry - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Flow Cytometry analysis of 293T cells labelling Apolipoprotein E with purified ab51015 at 1/20 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/1000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/10000 dilution (purified) + Human serum at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/10000 dilution (unpurified) + Human serum at 10 µg

Secondary
Goat anti-rabbit HRP labelled at 1/2000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Apolipoprotein E with purified ab51015 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

ab51015 (purified) at 1/30 immunoprecipitating Apolipoprotein in human fetal liver tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Anti-Apolipoprotein E antibody [EP1373Y] (ab51015)

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