Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1628Y] to Cytokeratin 8
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]
    See all Cytokeratin 8 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1628Y] to Cytokeratin 8

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human Cytokeratin 8 aa 300-400 (C terminal). The exact sequence is proprietary.
    Database link: P05787

  • Positive control

    • ICC/IF: HeLa cells Flow Cyt: HeLa cells

  • General notes

    Parent antibody ab53280 is KO validated.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)
    Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)

    Immunofluorescence staining of cytokeratin 8 in HeLa cells using ab192467. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192647 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) (ab150120) at 2 μg/ml (shown in pseudo-color red).

    Nuclear DNA was labeled in blue with DAPI.

    This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

    Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)

  • Flow Cytometry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)
    Flow Cytometry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)

    Flow cytometry analysis of HeLa cells stained with ab192467. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192467, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)
    Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467) Rosenbluth J et al., Nat Commun, 11(1), 1711. Fig 1c.; doi: 10.1038/s41467-020-15548-7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunofluorescence staining in human mammary organoids of cytokeratin 8 using ab192467 (green), cytokeratin 14 using ab206100 (red), and actin (white). Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with 0.5% Triton X-100 for 10 minutes at 4 °C. Primary antibodies incubated overnight at 4 °C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 µm. Organoids were imaged by confocal microscopy.

     

  • Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)
    Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)

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