Anti-Cytokeratin 8 antibody (ab131514)
Key features and details
- Rabbit polyclonal to Cytokeratin 8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 8 antibody
See all Cytokeratin 8 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide conjugated to KLH, derived from around amino acids 72-76 (
LLSPL
) of Human Cytokeratin 8 (NP_002264.1). -
Positive control
- HeLa cells; HUVEC and 293 cell extracts; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab131514)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cytokeratin 8 with ab131514 at 1/50 dilution (left) or ab131514 preincubated with blocking peptide (right).
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cytokeratin 8 with ab131514 at 1/100 dilution.
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All lanes : Anti-Cytokeratin 8 antibody (ab131514) at 1/500 dilution
Lane 1 : HUVEC extract
Lane 2 : 293 cell extract
Predicted band size: 53 kDa