Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JNK3 antibody [EPR5493(2)] (ab126591)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5493(2)] to JNK3
  • Suitable for: ELISA, WB, Flow Cyt, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-JNK3 antibody [EPR5493(2)]
    See all JNK3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5493(2)] to JNK3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human JNK3 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, SH-SY5Y and Human heart lysates; 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    Western blot - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    All lanes : Anti-JNK3 antibody [EPR5493(2)] (ab126591) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Human heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa

  • Flow Cytometry - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    Flow Cytometry - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    Overlay histogram showing SHSY-5Y cells stained with ab126591 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126591, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunocytochemistry/ Immunofluorescence - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    Immunocytochemistry/ Immunofluorescence - Anti-JNK3 antibody [EPR5493(2)] (ab126591)

    ab126591 at 1/100 dilution staining JNK3 in 293 cells by immunofluorescence.

  • ELISA - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    ELISA - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    ELISA analysis of Human JNK3 recombinant protein at 1000 ng/mL with ab126591. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • OI-RD Scanning - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    OI-RD Scanning - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-JNK3 antibody [EPR5493(2)] (ab126591)
    Anti-JNK3 antibody [EPR5493(2)] (ab126591)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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