Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)
![Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-391294-anti-sigma1receptor-antibody-epr2326669-western-blot-hek293t.jpg "Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23266-69] to Sigma1-receptor
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human
Overview
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Product name
Anti-Sigma1-receptor antibody [EPR23266-69]
See all Sigma1-receptor primary antibodies -
Description
Rabbit monoclonal [EPR23266-69] to Sigma1-receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentFlow Cyt HumanIHC-P RatHumanWB RatHumanRecombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Caco-2, A375, U-87 MG, HEK-293 and HeLa whole cell lysates; HEK-293T transfected with Sigma1-receptor whole cell lysate; Rat brain, hippocampus, lung and kidney tissue lysates. IHC-P: Human spinal cord and liver tissue; Rat spinal cord and liver tissue. Flow Cyt: HT-29 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23266-69 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)All lanes : Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with Sigma1-receptor (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3.25 seconds
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![Western blot - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-391293-anti-sigma1receptor-antibody-epr2326669-western-blot-rat-brain-tissue-lysate-20-ug.jpg)
Western blot - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)All lanes : Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat hippocampus tissue lysate
Lane 3 : Rat lung tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
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![Western blot - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-391292-anti-sigma1receptor-antibody-epr2326669-western-blot-caco2.jpg)
Western blot - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)All lanes : Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192) at 1/1000 dilution
Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : A375 (human malignant melanoma epithelial cell), whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
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![Flow Cytometry - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-5-anti-sigma1receptor-antibody-epr2326669-flow-cytometry-ht29.jpg)
Flow Cytometry - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling Sigma1-receptor with ab253192 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-4-anti-sigma1receptor-antibody-epr2326669-immunohistochemistry-rat-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sigma1-receptor with ab253192 at 1/500 dilution (1.23 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver (PMID: 8954936). The section was incubated with ab253192 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-3-anti-sigma1receptor-antibody-epr2326669-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Sigma1-receptor with ab253192 at 1/500 dilution (1.23 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver (PMID: 8954936). The section was incubated with ab253192 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-2-anti-sigma1receptor-antibody-epr2326669-immunohistochemistry-rat-spinal-cord.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)Immunohistochemical analysis of paraffin-embedded Rat spinal cord tissue labeling Sigma1-receptor with ab253192 at 1/500 dilution (1.23 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat spinal cord (PMID: 23314020). The section was incubated with ab253192 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-1-anti-sigma1receptor-antibody-epr2326669-immunohistochemistry-human-spinal-cord.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)Immunohistochemical analysis of paraffin-embedded Human spinal cord tissue labeling Sigma1-receptor with ab253192 at 1/500 dilution (1.23 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spinal cord (PMID: 23314020). The section was incubated with ab253192 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)](https://www.abcam.com/ps/products/253/ab253192/Images/ab253192-9-benefits-of-recombinant-antibodies.png)
Anti-Sigma1-receptor antibody [EPR23266-69] (ab253192)