ab194399 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab194399 at 1µg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Cytokeratin 19 antibody [A53-B] (ab194399) at 2 µg/ml + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Predicted band size: 44 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Cytokeratin 19 with ab194399 at 2 µg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Cytokeratin 19 with ab194399 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (cyan). The nuclear counterstain is Reddot (red).

All lanes : Anti-Cytokeratin 19 antibody [A53-B] (ab194399) at 2 µg/ml

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 44 kDa