Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR127B] to Cytokeratin 19
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-Cytokeratin 19 antibody [EPNCIR127B]
See all Cytokeratin 19 primary antibodies -
Description
Rabbit monoclonal [EPNCIR127B] to Cytokeratin 19 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse -
Immunogen
Synthetic peptide corresponding to Mouse Cytokeratin 19 aa 350 to the C-terminus (C terminal).
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Positive control
- Mouse kidney, Mouse heart, Mouse spleen, and F9 cell lysates, Mouse colon tissue
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General notes
This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Terry Van Dyke. View antibodies from NCI Center for Cancer Research Collaboration.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR127B -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Cytokeratin 19 with purified ab133496 at 1:500 dilution (1.33 ?g/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) at 1/1000 dilution (Purified)
Lane 1 : Mouse kidney lysates
Lane 2 : Mouse heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 44 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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Immunohistochemical analysis of mouse orofacial tissue, staining Cytokeratin 19 with unpurified ab133496.
Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/500 in diluent) for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG (1/2) was used as the secondary antibody. -
Immunohistochemical analysis of paraffin embedded Mouse colon tissue labelling Cytokeratin 19 using unpurified ab133496 antibody at a dilution of 1/100.
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All lanes : Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) at 1/1000 dilution (unpurified)
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : F9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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