Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)

Key features and details

  • Mouse monoclonal [VU-1D9] to EpCAM - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-EpCAM antibody [VU-1D9] - BSA and Azide free
    See all EpCAM primary antibodies

  • Description

    Mouse monoclonal [VU-1D9] to EpCAM - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Rat, Ferret

  • Immunogen

    Tissue, cells or virus corresponding to Human EpCAM. Immunogen from small cell lung carcinoma cells.
    Database link: P16422

  • Positive control

    • Flow Cyt: A431 and HT29 cells. IHC-P: Human colon tissue. ICC: T47D and A431 cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    ab212579 is purified from Bioreactor Concentrate by Protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    VU-1D9

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    Flow Cytometry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)

    This data was developed using the same antibody clone in a different buffer formulation (ab212579)

    Flow cytometry overlay histogram showing wild-type A431 (green line) and EPCAM knockout A431 cells stained with ab187372 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab187372) (1x106 in 100μl at 0.2 μg/ml) for 30 min at 4°C.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.

    Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type A431 - black line; EPCAM knockout A431 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)

    Immunohistochemical analysis of paraffin embedded, formalin fixed Human colon tissue labeling EpCAM using ab212579.

  • Immunocytochemistry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    Immunocytochemistry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)

    This data was developed using the same antibody clone in a different buffer formulation (ab187372). ab187372 staining EpCAM in wild-type A431 cells (top panel) and EpCAM knockout A431 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab187372 at 0.5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunocytochemistry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    Immunocytochemistry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    This data was developed using the same antibody clone in a different buffer formulation (ab187372). ab187372 staining EpCAM in T47D positive cells (top panel) and HeLa negative cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab187372 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI. This antibody performed similarly using 100% methanol fixation. Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.
  • Immunocytochemistry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    Immunocytochemistry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    This data was developed using the same antibody clone in a different buffer formulation (ab187372). ab187372 staining EpCAM in wild-type A431 cells (top panel) and EpCAM knockout A431 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab187372 at 0.1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI. This antibody performed similarly using 100% methanol fixation.
    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.
  • Flow Cytometry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
    Flow Cytometry - Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)

    Flow cytometric analysis of HT29 cells labeling EpCAM with ab212579 (red) at 1 µg/106 cells compared with an isotype control (green).

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