Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)
![Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)](https://www.abcam.com/ps/products/228/ab228856/Images/ab228856-309448-anti-cd8-alpha-antibody-epr20305-immunohistochemistry.jpg "Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20305] to CD8 alpha - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free
See all CD8 alpha primary antibodies -
Description
Rabbit monoclonal [EPR20305] to CD8 alpha - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lymph node, thymus and spleen lysates. IHC-P: Mouse spleen and thymus tissues.
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General notes
Ab228856 is the carrier-free version of ab209775. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab228856 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20305 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)
This IHC data was generated using the same anti-CD8 alpha antibody clone, EPR20305, in a different buffer formulation (cat# ab209775).
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on mouse thymus. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)](https://www.abcam.com/ps/products/228/ab228856/Images/ab228856-309447-anti-cd8-alpha-antibody-epr20305-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)This IHC data was generated using the same anti-CD8 alpha antibody clone, EPR20305, in a different buffer formulation (cat# ab209775).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on T cells around peripheral artery lymphatic sheath of mouse spleen (PMID: 23482450). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)](https://www.abcam.com/ps/products/228/ab228856/Images/ab228856-3-benefits-of-recombinant-antibodies.png)
Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)
