Anti-CD8 alpha antibody [OX-8] - BSA and Azide free (ab244566)
![Anti-CD8 alpha antibody [OX-8] - BSA and Azide free (ab244566)](https://www.abcam.com/ps/products/244/ab244566/Images/ab244566-352440-anti-cd8-alpha-pbs-only-carrier-free-western-blot-thymus-brain-rat.jpg "Anti-CD8 alpha antibody [OX-8] - BSA and Azide free (ab244566)")
Key features and details
- Mouse monoclonal [OX-8] to CD8 alpha - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD8 alpha antibody [OX-8] - BSA and Azide free
See all CD8 alpha primary antibodies -
Description
Mouse monoclonal [OX-8] to CD8 alpha - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Tissue, cells or virus. High molecular weight rat thymocyte glycoproteins
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Positive control
- WB: Rat thymus tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-CD8 alpha antibody [OX-8] - BSA and Azide free (ab244566)All lanes : Anti-CD8 alpha antibody [OX-8] (ab33786) at 1 µg/ml
Lane 1 : Rat thymus tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 26 kDaThis image was produced using the same clone, but different formulation, ab33786.
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab33786 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
