Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD8 alpha antibody [EPR20305] (ab209775)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20305] to CD8 alpha
Suitable for: IHC-P, WB
Reacts with: Mouse

Product name

Anti-CD8 alpha antibody [EPR20305]
See all CD8 alpha primary antibodies
Description

Rabbit monoclonal [EPR20305] to CD8 alpha
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lymph node, thymus and spleen lysates. IHC-P: Mouse spleen and thymus tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20305
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] (ab209775)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on T cells around peripheral artery lymphatic sheath of mouse spleen (PMID: 23482450). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] (ab209775)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on mouse thymus. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD8 alpha antibody [EPR20305] (ab209775)

All lanes : Anti-CD8 alpha antibody [EPR20305] (ab209775) at 1/1000 dilution

Lane 1 : Mouse lymph node lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 27 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.
Anti-CD8 alpha antibody [EPR20305] (ab209775)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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