Anti-Cathepsin W antibody [EPR15135] (ab191083)
![Anti-Cathepsin W antibody [EPR15135] (ab191083)](https://www.abcam.com/ps/products/191/ab191083/Images/ab191083-228212-anti-cathepsin-w-antibody-epr15135-western-blot.jpg "Anti-Cathepsin W antibody [EPR15135] (ab191083)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15135] to Cathepsin W
- Suitable for: IP, WB
- Reacts with: Rat, Human
Overview
-
Product name
Anti-Cathepsin W antibody [EPR15135]
See all Cathepsin W primary antibodies -
Description
Rabbit monoclonal [EPR15135] to Cathepsin W -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB RatHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HuT 78, MOLT4, Jurkat, HeLa, A431, SH-SY5Y, PC12 and K562 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15135 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083)All lanes : Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/50000 dilution
Lane 1 : HuT 78 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 42 kDa
-
![Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] (ab191083)](https://www.abcam.com/ps/products/191/ab191083/Images/ab191083-228215-anti-cathepsin-w-antibody-epr15135-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] (ab191083)Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab191083 at 1/30 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083)](https://www.abcam.com/ps/products/191/ab191083/Images/ab191083-228214-anti-cathepsin-w-antibody-epr15135-western-blot.jpg)
Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083)Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/10000 dilution + PC12 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 42 kDa
-
![Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083)](https://www.abcam.com/ps/products/191/ab191083/Images/ab191083-228213-anti-cathepsin-w-antibody-epr15135-western-blot.jpg)
Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083)All lanes : Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/20000 dilution
Lane 1 : A431 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 42 kDa
-
![Anti-Cathepsin W antibody [EPR15135] (ab191083)](https://www.abcam.com/ps/products/191/ab191083/Images/ab191083-5-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin W antibody [EPR15135] (ab191083)
