FITC Anti-CD8 alpha antibody [EPR21769] (ab237367)
![FITC Anti-CD8 alpha antibody [EPR21769] (ab237367)](https://www.abcam.com/ps/products/237/ab237367/Images/ab237367-1-ab237367FLOW1.jpg "FITC Anti-CD8 alpha antibody [EPR21769] (ab237367)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EPR21769] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Overview
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Product name
FITC Anti-CD8 alpha antibody [EPR21769]
See all CD8 alpha primary antibodies -
Description
FITC Rabbit monoclonal [EPR21769] to CD8 alpha -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment within Mouse CD8 alpha aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01731 -
Positive control
- Flow Cyt: C57BL/6 mouse splenocytes.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21769 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - FITC Anti-CD8 alpha antibody [EPR21769] (ab237367)
C57BL/6 mouse splenocytes stained with ab237367 (right) or Rabbit IgG FITC (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab237367) or Rabbit IgG FITC Isotype (1x106 in 100 µl at 1:500 (1 µg/ml)) for 30 mins on ice. The cells were simultaneously stained with CD4 PerCP conjugated antibody.
Acquisition of >50,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
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![FITC Anti-CD8 alpha antibody [EPR21769] (ab237367)](https://www.abcam.com/ps/products/237/ab237367/Images/ab237367-2-benefits-of-recombinant-antibodies.png)
FITC Anti-CD8 alpha antibody [EPR21769] (ab237367)