Anti-4-1BBL antibody [014] - BSA and Azide free (ab277140)
![Anti-4-1BBL antibody [014] - BSA and Azide free (ab277140)](https://www.abcam.com/ps/products/277/ab277140/Images/ab277140-2-Anti41BBL-antibody-Flow-cyt-Raw2647-cells.jpg "Anti-4-1BBL antibody [014] - BSA and Azide free (ab277140)")
Key features and details
- Rabbit monoclonal [014] to 4-1BBL - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-4-1BBL antibody [014] - BSA and Azide free
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [014] to 4-1BBL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC Mouse
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Immunogen
Recombinant fragment corresponding to Mouse 4-1BBL aa 104-309. Genbank: NP_033430.1
Database link: P41274 -
Positive control
- Flow cyt: RAW264.7 cells ICC: RAW264.7 cells.
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General notes
4-1BBL is an alternative name for TNFSF9.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
014 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-4-1BBL antibody (ab277140)
Flow cytometric analysis of 4-1BBL expression on Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were stained with ab277140 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also staibed with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Immunocytochemistry - Anti-4-1BBL antibody (ab277140)Confocal immunofluorescence analysis of 4-1BBL in RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277140 at 1/100 dilution at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).