Bovine Serum Albumin (nitrated ) (ab131379)
Key features and details
- Expression system: Native
- Purity: > 96% SDS-PAGE
- Suitable for: SDS-PAGE, WB
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Product name
Bovine Serum Albumin (nitrated )
See all Bovine Serum Albumin proteins and peptides -
Purity
> 96 % SDS-PAGE. -
Expression system
Native -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Cow -
Sequence
MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIA FSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCK VASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEF KADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGAC LLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVARLSQKFPKAE FVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKE CCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFL GSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKL KHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVS RSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCC TESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQT ALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVV STQTALA -
Predicted molecular weight
69 kDa -
Amino acids
1 to 607
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.61% Tris HCl, 15% Glycerol, 0.87% Sodium chloride
Images
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Western blot - Bovine Serum Albumin (nitrated ) (ab131379)All lanes: Anti-Nitrotyrosine antibody (ab110282) 0.4 µg/mL.
Lane 1: Molecular weight marker
Lane 2: BSA 100 ng
Lane 3: BSA 25 ng
Lane 4: Nitrated BSA 100 ng
Lane 5: Nitrated BSA 50 ng
Lane 6: Nitrated BSA 25 ng
Lane 7: Nitrated BSA 12.5 ng
Lane 8: Nitrated BSA 6.25 ng
Lane 9: Nitrated BSA 3.125 ngSecondary antibody:
Goat polyclonal to Mouse IgG – Pre-Adsorbed (HRP) at 1/6000
developed using the ECL technique.Performed under reducing conditions.
Predicted band size: 69,293 Da
Observed band size: 69 kDaExposure time: 5 minutes
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SDS-PAGE - Bovine Serum Albumin (nitrated ) (ab131379)Lane 1: Nitrated Bovine Serum Albumin protein (50 ng)
Lane 2: Molecular weight marker
Lane 3: Bovine Serum Albumin protein (50 ng)Gel: 4-20% acrylamide separation gel
Predicted band size: 69 kDa
