Anti-MT4-MMP antibody [EP1270Y] (ab51075)
![Anti-MT4-MMP antibody [EP1270Y] (ab51075)](https://www.abcam.com/ps/products/51/ab51075/Images/ab51075-62977-anti-mt4-mmp-antibody-ep1270y-western-blot.jpg "Anti-MT4-MMP antibody [EP1270Y] (ab51075)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1270Y] to MT4-MMP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MT4-MMP antibody [EP1270Y]
See all MT4-MMP primary antibodies -
Description
Rabbit monoclonal [EP1270Y] to MT4-MMP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Mouse
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Immunogen
Synthetic peptide within Human MT4-MMP aa 150-250 (internal sequence). The exact sequence is proprietary.
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Positive control
- Mouse brain lysate, human lymphoma tissue or HeLa cells.
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General notes
Protein previously labeled as MMP17.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1270Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MT4-MMP antibody [EP1270Y] (ab51075)Anti-MT4-MMP antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] (ab51075)](https://www.abcam.com/ps/products/51/ab51075/Images/ab51075-62976-anti-mt4-mmp-antibody-ep1270y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] (ab51075)ab51075 (1:50) staining human MT4-MMP in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] (ab51075)](https://www.abcam.com/ps/products/51/ab51075/Images/ab51075-62975-anti-mt4-mmp-antibody-ep1270y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] (ab51075)Immunofluorescent staining of HeLa cells using ab51075 (1:50). -
![Anti-MT4-MMP antibody [EP1270Y] (ab51075)](https://www.abcam.com/ps/products/51/ab51075/Images/ab51075-5-benefits-of-recombinant-antibodies.png)
Anti-MT4-MMP antibody [EP1270Y] (ab51075)
