Recombinant human MMP3 protein (ab134448)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, Inhibition Assay
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Product name
Recombinant human MMP3 protein
See all MMP3 proteins and peptides -
Biological activity
The specific activity of ab134448 was >1000 mU/mg when assayed with the synthetic substrate (7-methoxycoumarin-4yl) acetyl-Arg-Pro-Lys-Pro-ValGlu-Nva-Trp-Arg-Lys (Dnp) - NH2. -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
22 kDa -
Amino acids
100 to 273
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Preparation and Storage
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Alternative names
- CHDS6
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
see all -
Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
Images
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SDS-PAGE - Recombinant human MMP3 protein (ab134448)SDS-PAGE analysis of ab134448; reducing conditions (left), and zymogram (non-reducing) right.
