Anti-MMP10 antibody (ab38930)
Key features and details
- Rabbit polyclonal to MMP10
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP10 antibody
See all MMP10 primary antibodies -
Description
Rabbit polyclonal to MMP10 -
Host species
Rabbit -
Specificity
The antibody binds to the hinge region of MMP10. The antibody binds to Stromelysin 2, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc.). The antibody also recognizes the cleaved active enzyme and will also bind to the non-reduced protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide based on the hinge region of the human MMP10 sequence.
Read Abcam's proprietary immunogen policy (Peptide available as ab41075.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP10 antibody (ab38930)Ab38930 staining Human normal trachea. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
