Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1247Y] to MMP1 - Low endotoxin, Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free
    See all MMP1 primary antibodies

  • Description

    Rabbit monoclonal [EP1247Y] to MMP1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human testis and cervical carcinoma tissues. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HeLa cells.

  • General notes

    ab215979 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979) This image is courtesy of an anonymous Abreview.

    Formaldehyde-fixed, paraffin-embedded human placenta tissue stained for MMP1 using ab52631 at 1/40 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of cervix tissue labeling MMP1 with ab52631 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with unpurified ab52631 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with purified ab52631 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/30. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/30) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Flow cytometry analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Flow cytometry analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). Green - Isotype control, rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling MMP1 with ab52631 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

    ICC/IF image of unpurified ab52631 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52631, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

  • Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
    Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

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