Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1249Y] to MMP1
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-MMP1 antibody [EP1249Y]
See all MMP1 primary antibodies
Description

Rabbit monoclonal [EP1249Y] to MMP1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MMP1 aa 1-100 (N terminal). The exact sequence is proprietary. The immunogen amino acid sequence is within the propeptide sequence.
Positive control
- A431 cell lysate
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1249Y
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs

Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)

All lanes : Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/500 dilution

Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate
Lane 2 : Lysate from HeLa cells untreated with TPA
Lane 3 : HeLa treated with 100 ng/mL TPA for 10 hours cell lysate
Lane 4 : Human colon cancer
Lane 5 : Human breast cancer

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 30 seconds

Blocking and dilution buffer: 5% NFDM/TBST
Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)

Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/1000 dilution + A431 cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa
Anti-MMP1 antibody [EP1249Y] (ab134184)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com