Anti-MMP1 antibody [EP1249Y] (ab134184)
![Anti-MMP1 antibody [EP1249Y] (ab134184)](https://www.abcam.com/ps/products/134/ab134184/Images/ab134184-258975-anti-mmp1-antibody-ep1249y-western-blot.jpg "Anti-MMP1 antibody [EP1249Y] (ab134184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1249Y] to MMP1
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-MMP1 antibody [EP1249Y]
See all MMP1 primary antibodies -
Description
Rabbit monoclonal [EP1249Y] to MMP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human MMP1 aa 1-100 (N terminal). The exact sequence is proprietary. The immunogen amino acid sequence is within the propeptide sequence.
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Positive control
- A431 cell lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1249Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)All lanes : Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/500 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate
Lane 2 : Lysate from HeLa cells untreated with TPA
Lane 3 : HeLa treated with 100 ng/mL TPA for 10 hours cell lysate
Lane 4 : Human colon cancer
Lane 5 : Human breast cancer
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)](https://www.abcam.com/ps/products/134/ab134184/Images/ab134184-148508-anti-mmp1-antibody-ep1249y-western-blot.jpg)
Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/1000 dilution + A431 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
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![Anti-MMP1 antibody [EP1249Y] (ab134184)](https://www.abcam.com/ps/products/134/ab134184/Images/ab134184-3-benefits-of-recombinant-antibodies.png)
Anti-MMP1 antibody [EP1249Y] (ab134184)
