Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MyD88 antibody [EPR21824] (ab219413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21824] to MyD88
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MyD88 antibody [EPR21824]
    See all MyD88 primary antibodies

  • Description

    Rabbit monoclonal [EPR21824] to MyD88

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. HepG2 and Ramos whole cell lysate. Rat and mouse liver lysate. RAW 264.7 and A20 whole cell lysate. Mouse lung lysate. IP: RAW 264.7 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MyD88 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EPR21824] to MyD88 (ab219413) at 1/100000 dilution

    Predicted band size: 33 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab219413 was shown to specifically react with MyD88 in wild-type HAP1 cells as signal was lost in MyD88 knockout cells. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab219413 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1 : Rat liver lysate
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : A20 (Mouse reticulum sarcoma B lymphocyte) cell lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Mouse lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)
    Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)

    MyD88 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab219413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: RAW 264.7 whole cell lysate 10 µg (Input).
    Lane 2: ab219413 IP in RAW 264.7 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219413 in RAW 264.7 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Anti-MyD88 antibody [EPR21824] (ab219413)
    Anti-MyD88 antibody [EPR21824] (ab219413)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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