Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MyD88 antibody [EPR21824] (ab219413)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21824] to MyD88
Suitable for: IP, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-MyD88 antibody [EPR21824]
See all MyD88 primary antibodies
Description

Rabbit monoclonal [EPR21824] to MyD88
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HAP1 whole cell lysate. HepG2 and Ramos whole cell lysate. Rat and mouse liver lysate. RAW 264.7 and A20 whole cell lysate. Mouse lung lysate. IP: RAW 264.7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21824
Isotype

IgG
Research areas

Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)

All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MyD88 knockout HAP1 whole cell lysate
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Rabbit monoclonal [EPR21824] to MyD88 (ab219413) at 1/100000 dilution

Predicted band size: 33 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

ab219413 was shown to specifically react with MyD88 in wild-type HAP1 cells as signal was lost in MyD88 knockout cells. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab219413 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique
Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)

All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

Lane 1 : Rat liver lysate
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : A20 (Mouse reticulum sarcoma B lymphocyte) cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 33 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)

MyD88 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab219413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: RAW 264.7 whole cell lysate 10 µg (Input).
Lane 2: ab219413 IP in RAW 264.7 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219413 in RAW 264.7 whole cell lysate (-).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Anti-MyD88 antibody [EPR21824] (ab219413)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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