Anti-MyD88 antibody (ab28763)
Key features and details
- Goat polyclonal to MyD88
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MyD88 antibody
See all MyD88 primary antibodies -
Description
Goat polyclonal to MyD88 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
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Immunogen
Synthetic peptide:
C-IKYKAM KKEFP
, corresponding to Internal sequence amino acids 255-265 of Human MyD88. -
Positive control
- Human T cell line MOLT4 cell lysate. Human thymus lysate. Human tonsil tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MyD88 antibody (ab28763)Anti-MyD88 antibody (ab28763) at 0.2 µg/ml + Human thymus lysate at 35 µg
Predicted band size: 33 kDa
Observed band size: 33 kDaPrimary antibody incubated for 1 hour and detected using chemiluminescence.
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Western blot - Anti-MyD88 antibody (ab28763)Anti-MyD88 antibody (ab28763) at 0.01 µg/ml + Human T cell line MOLT4 cell lysate, 30µg total protein per lane
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (ab28763)ab28763 (4µg/ml) staining of paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
