Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MyD88 antibody [EPR21824] - BSA and Azide free (ab236766)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21824] to MyD88 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MyD88 antibody [EPR21824] - BSA and Azide free
    See all MyD88 primary antibodies

  • Description

    Rabbit monoclonal [EPR21824] to MyD88 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. HepG2 and Ramos whole cell lysate.

  • General notes

    Ab236766 is the carrier-free version of ab219413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236766 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-MyD88 antibody [EPR21824] - BSA and Azide free (ab236766)
    Immunoprecipitation - Anti-MyD88 antibody [EPR21824] - BSA and Azide free (ab236766)

    MyD88 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab219413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: RAW 264.7 whole cell lysate 10 µg (Input).
    Lane 2: ab219413 IP in RAW 264.7 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219413 in RAW 264.7 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219413).

  • Western blot - Anti-MyD88 antibody [EPR21824] - BSA and Azide free (ab236766)
    Western blot - Anti-MyD88 antibody [EPR21824] - BSA and Azide free (ab236766)
    All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MyD88 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab219413 was shown to specifically react with MyD88 in wild-type HAP1 cells as signal was lost in MyD88 knockout cells. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab219413 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219413).

  • Anti-MyD88 antibody [EPR21824] - BSA and Azide free (ab236766)
    Anti-MyD88 antibody [EPR21824] - BSA and Azide free (ab236766)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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