Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7908] to 58K Golgi protein - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free
    See all 58K Golgi protein primary antibodies

  • Description

    Rabbit monoclonal [EPR7908] to 58K Golgi protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248399 is the carrier-free version of ab131275 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    Flow Cytometry - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    This data was developed using ab131275, the same antibody clone in a different buffer formulation.Overlay histogram showing HepG2 cells stained with ab131275 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131275, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    Western blot - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    Anti-58K Golgi protein antibody [EPR7908] (ab131275) at 1/1000 dilution + Human fetal liver tissue lysate

    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 58 kDa



    This data was developed using ab131275, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    This data was developed using ab131275, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelled with ab131275 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    This data was developed using ab131275, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labelled with ab131275 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    OI-RD Scanning - Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    This data was developed using ab131275, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)
    Anti-58K Golgi protein antibody [EPR7908] - BSA and Azide free (ab248399)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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