Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-58K Golgi protein antibody (ab133016)

Key features and details

  • Rabbit polyclonal to 58K Golgi protein
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-58K Golgi protein antibody
    See all 58K Golgi protein primary antibodies

  • Description

    Rabbit polyclonal to 58K Golgi protein

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Gorilla

  • Immunogen

    Synthetic peptide corresponding to Human 58K Golgi protein aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156862)

  • Positive control

    • This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human Liver; Human Fetal Liver; Mouse Liver and Rat Liver. This antibody also gave a positive signal within IF in the following Methanol fixed cell line: HeLa.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab133016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.

  • Pathway

    Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.

  • Involvement in disease

    Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

  • Sequence similarities

    In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.

  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    see all

Images

  • Western blot - Anti-58K Golgi protein antibody (ab133016)
    Western blot - Anti-58K Golgi protein antibody (ab133016)
    All lanes : Anti-58K Golgi protein antibody (ab133016) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Human) Tissue Lysate - fetal tissue
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Liver (Mouse) Tissue Lysate
    Lane 5 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa
    Additional bands at: 27 kDa, 38 kDa, 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133016 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody (ab133016)
    Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody (ab133016)
    ab133016 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab133016 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References (0)

Publishing research using ab133016? Please let us know so that we can cite the reference in this datasheet.

ab133016 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-58K Golgi protein antibody (ab133016)
    Western blot - Anti-58K Golgi protein antibody (ab133016)
    All lanes : Anti-58K Golgi protein antibody (ab133016) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Human) Tissue Lysate - fetal tissue
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Liver (Mouse) Tissue Lysate
    Lane 5 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa
    Additional bands at: 27 kDa, 38 kDa, 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133016 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody (ab133016)
    Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody (ab133016)
    ab133016 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab133016 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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