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Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

Key features and details

  • Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-58K Golgi protein antibody [58K-9] - Golgi Marker
    See all 58K Golgi protein primary antibodies

  • Description

    Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    corresponding to 58K Golgi protein.

  • Positive control

    • Rat Liver and HeLa cells. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
    Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

    ab27043 staining 58K Golgi protein - Golgi Marker in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab27043 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
    Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
    ab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red).
  • Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
    Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
    All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
    Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa


    Exposure time: 3 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Flow Cytometry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
    Flow Cytometry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
    Overlay histogram showing HepG2 cells stained with ab27043 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27043, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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