Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
![Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.com/ps/products/27/ab27043/Images/ab27043-20-anti-58k-golgi-protein-58k9-immunocytochemistry-antibody-hela-human.jpg "Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)")
Key features and details
- Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
- Suitable for: ICC, WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-58K Golgi protein antibody [58K-9] - Golgi Marker
See all 58K Golgi protein primary antibodies -
Description
Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanWB Rat
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Immunogen
corresponding to 58K Golgi protein.
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Positive control
- Rat Liver and HeLa cells. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
58K-9 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
ab27043 staining 58K Golgi protein - Golgi Marker in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab27043 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.com/ps/products/27/ab27043/Images/ab27043-37831-anti-58k-golgi-protein-antibody-58k-9-golgi-marker-immunofluorescence.jpg)
Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red). -
![Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.com/ps/products/27/ab27043/Images/ab27043-37832-anti-58k-golgi-protein-antibody-58k-9-golgi-marker-western-blot.jpg)
Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 3 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
![Flow Cytometry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.com/ps/products/27/ab27043/Images/ab27043-37829-anti-58k-golgi-protein-antibody-58k-9-golgi-marker-flow-cytometry.jpg)
Flow Cytometry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)Overlay histogram showing HepG2 cells stained with ab27043 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27043, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
