Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)
![Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)](https://www.abcam.com/ps/products/223/ab223541/Images/ab223541-3-ab199949272075IF1.jpg "Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19527] to TMC1 - Low endotoxin, Azide free
- Suitable for: IHC-Fr, WB, ICC/IF, IP
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free
See all TMC1 primary antibodies -
Description
Rabbit monoclonal [EPR19527] to TMC1 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293T whole cell lysate transfected with DDDDK tagged mouse TMC1 . IHC-Fr: P20 mouse cochlea tissue. ICC/IF: Mouse TMC1-GFP transfected HEK-293T cells. IP: HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.
-
General notes
ab223541 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19527 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with mouse TMC1-GFP or GFP only, labeling TMC1 with ab199949 at 1/500 dilution, followed by Alexa Fluor® 594 Goat anti-Rabbit (ab150080) secondary antibody at 1/1000 dilution (red).
The results show cytoplasmic staining on mouse TMC1-GFP transfected HEK-293T cells.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.
The negative controls are as follows:
-ve control 1: ab199949 at 1/500 dilution followed by ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150080 (Alexa Fluor® 594 Goat anti-Rabbit secondary) at 1/1000 dilution.Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199949).
-
![Immunoprecipitation - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)](https://www.abcam.com/ps/products/223/ab223541/Images/ab223541-2-ab199949272074199949IP1.jpg)
Immunoprecipitation - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)TMC1 was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, transfected with pcDNA3.1-TMC1, with ab199949 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199949 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1, 10 μg (Input).
Lane 2: ab199949 IP in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199949 in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199949).
-
![Immunohistochemistry (Frozen sections) - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)](https://www.abcam.com/ps/products/223/ab223541/Images/ab223541-282795-ab199949IHCfr.jpg)
Immunohistochemistry (Frozen sections) - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)This image was made useing an alternate version of ab223541. This is the version with BSA and Azide ab199949.
Immunohistochemical analysis of 4% paraformaldehyde-fixed frozen P20 mouse cochlea tissue labeling TMC1 with ab199949 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (H+L) Dylight 488 secondary antibody at 1/500 dilution (green).
The results show specific staining on cochlear hair cells.
This data was kindly provided by our collaborator Dr. Yun Shi Nanjing University MARC.
This is wholemount IF with no permeabilisation or antigen retrieval.
-
![Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)](https://www.abcam.com/ps/products/223/ab223541/Images/ab223541-4-benefits-of-recombinant-antibodies.png)
Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (ab223541)
