Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)
![Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-391466-anti-protein-phosphatase-1-gamma-2-antibody-epr8934-western-blot-human-knockout-lysate.jpg "Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8934] to Protein phosphatase 1 gamma 2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934]
See all Protein phosphatase 1 gamma 2 primary antibodies -
Description
Rabbit monoclonal [EPR8934] to Protein phosphatase 1 gamma 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Protein phosphatase 1 gamma 2 aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T, HeLa, SKBR3 and T47D cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 4.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8934 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)All lanes : Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPP1CC knockout HeLa cell lysate
Lane 3 : T-47D cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab134947 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.
ab134947 Anti-Protein phosphatase 1 gamma 2 antibody was shown to specifically react with Protein phosphatase 1 gamma 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264875 (knockout cell lysate ab258131) was used. Wild-type and Protein phosphatase 1 gamma 2 knockout samples were subjected to SDS-PAGE. ab134947 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![OI-RD Scanning - Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-149154-anti-protein-phosphatase-1-gamma-2-antibody-epr8934.jpg)
OI-RD Scanning - Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947) -
![Western blot - Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-149155-anti-protein-phosphatase-1-gamma-2-antibody-epr8934-western-blot.jpg)
Western blot - Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)All lanes : Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SKBR3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
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![Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-4-benefits-of-recombinant-antibodies.png)
Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947)