All lanes : Anti-SAFB antibody [EPR13588] - C-terminal (ab187650) at 1/20000 dilution

Lane 1 : HeLa cell line lysate
Lane 2 : SH SY5Y cell line lysate
Lane 3 : 293 cell line lysate
Lane 4 : A431 cell line lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 103 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

This data was developed using ab187650, the same antibody clone in a different buffer formulation.

All lanes : Anti-SAFB antibody [EPR13588] - C-terminal (ab187650) at 1/1000 dilution

Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat brain tissue lysate

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 103 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

This data was developed using ab187650, the same antibody clone in a different buffer formulation.

This data was developed using ab187650, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAFB with ab187650 at 1/500 followed by ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG and counterstained with Hemotoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using ab187650, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded rat brain tissue labeling SAFB with ab187650 at 1/500 followed by ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG and counterstained with Hemotoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using ab187650, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling SAFB with ab187650 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counterstained with Dapi (blue).

This data was developed using ab187650, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling SAFB with ab187650 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counterstained with Dapi (blue).