Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Chk1S317-G1] to Chk1 (phospho S317)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human, Monkey

Product name

Anti-Chk1 (phospho S317) antibody [Chk1S317-G1]
See all Chk1 primary antibodies
Description

Rabbit monoclonal [Chk1S317-G1] to Chk1 (phospho S317)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Chk1 (phospho S317). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14757

Run BLAST with Run BLAST with
Positive control
- WB: COS7 (treated with UV + TPA) cell extract. Flow cyt: K562 and NIH/3T3 cells (both treated with IFNa + IL4 + pervanadate); K562 cells (treated with pervanadate).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

Chk1S317-G1
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or using 0.1 µg/mL of isotype control (orange) or treated with pervanadate and using 0.1 µg/mL of ab278717 (green) or pervanadate and blocked with phospho peptide (black) or pervanadate and blocked with non-phospho peptide (purple).
Western blot - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

All lanes : Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717) at 0.01 µg/ml

Lane 1 : COS7 cell extract treated with UV+ TPA
Lane 2 : COS7 cell extract untreated with UV+ TPA

Predicted band size: 54 kDa
Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or using 0.1 µg/mL of isotype control (orange) or treated with IFNa + IL4 + pervanadate and using 0.1 µg/mL of ab278717 (green) or IFNa + IL4 + Pv and blocked with phospho peptide (black) or IFNa + IL4 + Pv and blocked with non-phospho peptide (purple).
Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL4 + pervanadate (green) using ab278717 at 0.1 µg/mL.
Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

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