Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Chk1S317-G1] to Chk1 (phospho S317)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human, Monkey

Overview

  • Product name

    Anti-Chk1 (phospho S317) antibody [Chk1S317-G1]
    See all Chk1 primary antibodies

  • Description

    Rabbit monoclonal [Chk1S317-G1] to Chk1 (phospho S317)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Chk1 (phospho S317). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O14757

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: COS7 (treated with UV + TPA) cell extract. Flow cyt: K562 and NIH/3T3 cells (both treated with IFNa + IL4 + pervanadate); K562 cells (treated with pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or using 0.1 µg/mL of isotype control (orange) or treated with pervanadate and using 0.1 µg/mL of ab278717 (green) or pervanadate and blocked with phospho peptide (black) or pervanadate and blocked with non-phospho peptide (purple).
  • Western blot - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Western blot - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    All lanes : Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717) at 0.01 µg/ml

    Lane 1 : COS7 cell extract treated with UV+ TPA
    Lane 2 : COS7 cell extract untreated with UV+ TPA

    Predicted band size: 54 kDa

  • Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or using 0.1 µg/mL of isotype control (orange) or treated with IFNa + IL4 + pervanadate and using 0.1 µg/mL of ab278717 (green) or IFNa + IL4 + Pv and blocked with phospho peptide (black) or IFNa + IL4 + Pv and blocked with non-phospho peptide (purple).
  • Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Flow Cytometry - Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL4 + pervanadate (green) using ab278717 at 0.1 µg/mL.
  • Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
    Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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