Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)
![Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)](https://www.abcam.com/ps/products/240/ab240974/Images/ab240974-3-ab193360230930ab193360FC.jpg "Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15727] to RAB7B - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-RAB7B antibody [EPR15727] - BSA and Azide free
See all RAB7B primary antibodies -
Description
Rabbit monoclonal [EPR15727] to RAB7B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skeletal muscle and prostatic hyperplasia tissues. Flow Cyt: U937 cells.
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General notes
Ab240974 is the carrier-free version of ab193360. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15727 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)
Flow cytometric analysis of 2% paraformaldehyde-fixed U937 cells labeling RAB7B with ab193360 at 1/270 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193360) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)](https://www.abcam.com/ps/products/240/ab240974/Images/ab240974-2-ab193360230931ab193360IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193360)
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)](https://www.abcam.com/ps/products/240/ab240974/Images/ab240974-1-ab193360230932ab193360IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193360)
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)](https://www.abcam.com/ps/products/240/ab240974/Images/ab240974-4-benefits-of-recombinant-antibodies.png)
Anti-RAB7B antibody [EPR15727] - BSA and Azide free (ab240974)
