Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB7B antibody [EPR15727] (ab193360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15727] to RAB7B
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RAB7B antibody [EPR15727]
    See all RAB7B primary antibodies

  • Description

    Rabbit monoclonal [EPR15727] to RAB7B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • THP-1, 293, U937 and NIH/3T3 cell lysates; Human skeletal muscle and prostatic hyperplasia tissues; U937 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (ab193360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (ab193360)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RAB7B antibody [EPR15727] (ab193360)
    Western blot - Anti-RAB7B antibody [EPR15727] (ab193360)
    All lanes : Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : U937 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RAB7B antibody [EPR15727] (ab193360)
    Western blot - Anti-RAB7B antibody [EPR15727] (ab193360)
    Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (ab193360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (ab193360)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-RAB7B antibody [EPR15727] (ab193360)
    Flow Cytometry - Anti-RAB7B antibody [EPR15727] (ab193360)

    Flow cytometric analysis of 2% paraformaldehyde-fixed U937 cells labeling RAB7B with ab193360 at 1/270 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-RAB7B antibody [EPR15727] (ab193360)
    Anti-RAB7B antibody [EPR15727] (ab193360)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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