Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24159-81] to SS18-SSX1 Fusion Protein - BSA and Azide free
  • Suitable for: Dot blot, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free
    See all SS18-SSX1 Fusion Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR24159-81] to SS18-SSX1 Fusion Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IHC-P, Flow Cyt (Intra)more details
    Unsuitable for: ICC,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Dot Blot: Human SS18 + SSX(1/2/4) fusion peptide. IHC-P: Human synovial sarcoma tissue. HEK-293T transfected with SSXT-SSX2 labeled 6X His tag. Flow Cyt: HEK-293T transfected with myc-tagged SSX18-SSX2 fusion construct. HEK-293T transfected with myc tagged SS18+SSX1+SSX2+SSX4 construct.

  • General notes

    ab281565 is the carrier-free version of ab254406. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281565 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24159-81

  • Isotype

    IgG

Images

  • Dot Blot - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Dot Blot - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

    This image was produced using ab254406 the same clone but in a different formulation.

    Dot Blot - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406 used at a 1/1000 dilution (0.592 μg/ml)).

    Lane 1: Human SS18 + SSX(1/2/4) fusion peptide

    Lane 2: Human SS18 peptide

    Lane 3: Human SSX(1/2/4) peptide

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution.

    Exposure time is 3 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

    This image was produced using ab254406 the same clone but in a different formulation.

    Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Nuclear staining on human synovial sarcoma (PMID: 32141887).
    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Flow Cytometry - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Flow Cytometry - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

    This image was produced using ab254406 the same clone but in a different formulation.

    Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged SSX18-SSX2 fusion construct (right panel) stained for SS18-SSX1 Fusion Protein using ab254406 at a 1/5000 dilution (0.01 µg), followed by Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

    Isotype control (left panel) is Rabbit monoclonal IgG (ab172730).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

    This image was produced using ab254406 the same clone but in a different formulation.

    Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SSXT-SSX2 labeled 6X His tag and (Panel B) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid. labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Nuclear staining on (A) HEK-293T transfected with SSXT-SSX2 labeled 6X His tag, no staining on (B) HEK-293T transfected with an empty plasmid.
    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

    This image was produced using ab254406 the same clone but in a different formulation.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Negative control: No staining on human kidney.
    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

    This image was produced using ab254406 the same clone but in a different formulation.

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Negative control: No staining on human stomach.
    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Flow Cytometry - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Flow Cytometry - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

    This image was produced using ab254406 the same clone but in a different formulation.

    Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc tagged SS18+SSX1+SSX2+SSX4 construct (right panel) stained for SS18-SSX1 Fusion Protein using ab254406 at a 1/5000 dilution (0.01 µg), followed by Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

    Isotype control (left panel) is Rabbit monoclonal IgG (ab172730).

  • Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)
    Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] - BSA and Azide free (ab281565)

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