Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI1B4] to MyD88
Suitable for: WB, Flow Cyt, ICC/IF
Knockout validated
Reacts with: Human, African green monkey
Isotype: IgG2a

Product name

Anti-MyD88 antibody [OTI1B4]
See all MyD88 primary antibodies
Description

Mouse monoclonal [OTI1B4] to MyD88
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human MyD88.
Database link: Q99836
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY MyD88 cDNA; Jerkat, and MOLT4 cell lysates. Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpression plasmid; HeLa and Jurkat cells. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY MyD88; HeLa and Jurkat cells.
General notes

The clone number has been updated from 1B4 to OTI1B4, both clone numbers name the same clone.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI1B4
Isotype

IgG2a
Research areas

Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab119048 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.

ab119048 was shown to specifically react with MyD88 when MyD88 knockout samples were used. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. Ab119048 and ab176560 (loading control to alpha Tubulin) were diluted at 1/2000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 andGoat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)

All lanes : Anti-MyD88 antibody [OTI1B4] (ab119048) at 1/2000 dilution

Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY MyD88 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (ab119048)

ab119048 at 1/100 dilution staining MyD88 in COS7 cells transiently transfected with pCMV6-ENTRY MyD88 by Immunofluorescence.
Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)

ab119048 at 1/100 dilution, staining MyD88 in HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid(Red), or an empty vector control plasmid (Blue) by Flow Cytometry.
Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)

ab119048 at 1/100 dilution staining MyD88 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)

ab119048 at 1/100 dilution staining MyD88 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

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