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Anti-MyD88 antibody [OTI1B4] (ab119048)

Key features and details

  • Mouse monoclonal [OTI1B4] to MyD88
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human, African green monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-MyD88 antibody [OTI1B4]
    See all MyD88 primary antibodies

  • Description

    Mouse monoclonal [OTI1B4] to MyD88

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human MyD88.
    Database link: Q99836

  • Positive control

    • WB: HEK293T cell lysate transfected with pCMV6-ENTRY MyD88 cDNA; Jerkat, and MOLT4 cell lysates. Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpression plasmid; HeLa and Jurkat cells. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY MyD88; HeLa and Jurkat cells.

  • General notes

    The clone number has been updated from 1B4 to OTI1B4, both clone numbers name the same clone.

Properties

Images

  • Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)
    Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: Molt-4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab119048 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.

    ab119048 was shown to specifically react with MyD88 when MyD88 knockout samples were used. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE.  Ab119048 and ab176560 (loading control to alpha Tubulin) were diluted at 1/2000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 andGoat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)
    Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)
    All lanes : Anti-MyD88 antibody [OTI1B4] (ab119048) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY MyD88 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (ab119048)
    Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (ab119048)
    ab119048 at 1/100 dilution staining MyD88 in COS7 cells transiently transfected with pCMV6-ENTRY MyD88 by Immunofluorescence.
  • Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)
    Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)
    ab119048 at 1/100 dilution, staining MyD88 in HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid(Red), or an empty vector control plasmid (Blue) by Flow Cytometry.
  • Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)
    Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)
    ab119048 at 1/100 dilution staining MyD88 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)
    Flow Cytometry - Anti-MyD88 antibody [OTI1B4] (ab119048)
    ab119048 at 1/100 dilution staining MyD88 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

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