Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MyD88 antibody [4D6] (ab107585)

Key features and details

  • Mouse monoclonal [4D6] to MyD88
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-MyD88 antibody [4D6]
    See all MyD88 primary antibodies

  • Description

    Mouse monoclonal [4D6] to MyD88

  • Host species

    Mouse

  • Specificity

    Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).

  • Positive control

    • human ovary, human prostate and Jurkat cell lysates and CF-7 cells.

Properties

Images

  • Western blot - Anti-MyD88 antibody [4D6] (ab107585)
    Western blot - Anti-MyD88 antibody [4D6] (ab107585)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: MOLT4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
    ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-MyD88 antibody [4D6] (ab107585)
    Flow Cytometry - Anti-MyD88 antibody [4D6] (ab107585)
    Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)
    Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)
    Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
  • Western blot - Anti-MyD88 antibody [4D6] (ab107585)
    Western blot - Anti-MyD88 antibody [4D6] (ab107585)
    All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml

    Lane 1 : Human ovary cell lysate
    Lane 2 : Human prostate cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Jurkat cell lysate with Immunizing peptide

    Secondary
    All lanes : Goat anti-mouse Ig HRP

    Predicted band size: 33 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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