Anti-MyD88 antibody [4D6] (ab107585)
![Anti-MyD88 antibody [4D6] (ab107585)](https://www.abcam.com/ps/products/107/ab107585/Images/ab107585-266846-anti-myd88-antibody-4d6-western-blot.jpg "Anti-MyD88 antibody [4D6] (ab107585)")
Key features and details
- Mouse monoclonal [4D6] to MyD88
- Suitable for: WB, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MyD88 antibody [4D6]
See all MyD88 primary antibodies -
Description
Mouse monoclonal [4D6] to MyD88 -
Host species
Mouse -
Specificity
Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).
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Positive control
- human ovary, human prostate and Jurkat cell lysates and CF-7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4D6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MyD88 antibody [4D6] (ab107585)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MOLT4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry - Anti-MyD88 antibody [4D6] (ab107585)](https://www.abcam.com/ps/products/107/ab107585/Images/ab107585-120078-anti-myd88-antibody-4d6-flow-cytometry.jpg)
Flow Cytometry - Anti-MyD88 antibody [4D6] (ab107585)Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated. -
![Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)](https://www.abcam.com/ps/products/107/ab107585/Images/ab107585-120077-anti-myd88-antibody-4d6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom). -
![Western blot - Anti-MyD88 antibody [4D6] (ab107585)](https://www.abcam.com/ps/products/107/ab107585/Images/ab107585-120076-anti-myd88-antibody-4d6-western-blot.jpg)
Western blot - Anti-MyD88 antibody [4D6] (ab107585)All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml
Lane 1 : Human ovary cell lysate
Lane 2 : Human prostate cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Jurkat cell lysate with Immunizing peptide
Secondary
All lanes : Goat anti-mouse Ig HRP
Predicted band size: 33 kDa
