Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7459] to SYVN1/HRD1
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-SYVN1/HRD1 antibody [EP7459]
See all SYVN1/HRD1 primary antibodies -
Description
Rabbit monoclonal [EP7459] to SYVN1/HRD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: Q86TM6 -
Positive control
- 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
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General notes
This product was previously labelled as SYVN1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7459 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit. at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
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Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution
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Flow cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).
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