Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMUG1 antibody [EPR15624] (ab192240)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15624] to SMUG1
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SMUG1 antibody [EPR15624]
    See all SMUG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15624] to SMUG1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, 293 and MOLT4 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15624

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)
    Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)
    All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : SMUG1 knockout HAP1 cell lysate
    Lane 3 : MOLT-4 cell lysate
    Lane 4 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab192240 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

    ab192240 was shown to react with SMUG1 in wild-type HAP1 cells in western blot with loss of signal observed in SMUG1 knockout sample. Wild-type and SMUG1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab192240 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)
    Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)

    Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)
    Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)
    All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/5000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 30 kDa

  • Anti-SMUG1 antibody [EPR15624] (ab192240)
    Anti-SMUG1 antibody [EPR15624] (ab192240)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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