Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SMUG1 antibody [EPR15624] (ab192240)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15624] to SMUG1
Suitable for: IP, WB
Knockout validated
Reacts with: Human

Product name

Anti-SMUG1 antibody [EPR15624]
See all SMUG1 primary antibodies
Description

Rabbit monoclonal [EPR15624] to SMUG1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HAP1, 293 and MOLT4 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15624
Isotype

IgG
Research areas

Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)

All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/1000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SMUG1 knockout HAP1 cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 30 kDa
Observed band size: 30 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab192240 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab192240 was shown to react with SMUG1 in wild-type HAP1 cells in western blot with loss of signal observed in SMUG1 knockout sample. Wild-type and SMUG1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab192240 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)

Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)

All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/5000 dilution

Lane 1 : MOLT4 cell lysate
Lane 2 : 293 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 30 kDa
Anti-SMUG1 antibody [EPR15624] (ab192240)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com